Functional Expression of Drosophila para Sodium Channels

Functional Expression of Drosophila para Sodium Channels

The Drosophila para sodium channel alpha subunit was expressed in Xenopus oocytes alone and in combination with tipE, a putative Drosophila sodium channel accessory subunit. Coexpression of tipE with para results in elevated levels of sodium currents and accelerated current decay. Para/TipE sodium channels have biophysical and pharmacological properties similar to those of native channels. Howe...

Functional Expression of Drosophila para Sodium Channels Modulation by the Membrane Protein TipE and Toxin Pharmacology

The Drosophila para sodium channel a subunit was expressed in Xenopus oocytes alone and in combination with tipE , a putative Drosophila sodium channel accessory subunit. Coexpression of tipE with para results in elevated levels of sodium currents and accelerated current decay. Para/TipE sodium channels have biophysical and pharmacological properties similar to those of native channels. However...

Alterations in the expression and gating of Drosophila sodium channels by mutations in the para gene.

Mutations in the para gene specifically affect the expression of sodium currents in Drosophila. While 65% of wild-type embryonic neurons in culture express sodium currents, three distinct mutations in the para locus resulted in a decrease in the fraction of cells from which sodium currents could be recorded. This reduction was allele-dependent: macroscopic sodium currents were exhibited in 49% ...

functional expression of delayed rectifier potassium channels in cardiomyocytes ‎derivedfromembryonicstemcells

Activation of Drosophila Sodium Channels Promotes Modification by Deltamethrin

kdr and super-kdr are mutations in houseflies and other insects that confer 30- and 500-fold resistance to the pyrethroid deltamethrin. They correspond to single (L1014F) and double (L1014F+M918T) mutations in segment IIS6 and linker II(S4-S5) of Na channels. We expressed Drosophila para Na channels with and without these mutations and characterized their modification by deltamethrin. All wild-...